Genome-wide analysis of WRKY transcription factor genes in Toona sinensis: An insight into evolutionary characteristics and terpene synthesis

WRKY transcription factors (TFs), one of the largest TF families, serve critical roles in the regulation of secondary metabolite production. However, little is known about the expression pattern of WRKY genes during the germination and maturation processes of Toona sinensis buds. In the present study, the new assembly of the T. sinensis genome was used for the identification of 78 TsWRKY genes, including gene structures, phylogenetic features, chromosomal locations, conserved protein domains, cis-regulatory elements, synteny, and expression profiles. Gene duplication analysis revealed that gene tandem and segmental duplication events drove the expansion of the TsWRKYs family, with the latter playing a key role in the creation of new TsWRKY genes. The synteny and evolutionary constraint analyses of the WRKY proteins among T. sinensis and several distinct species provided more detailed evidence of gene evolution for TsWRKYs. Besides, the expression patterns and co-expression network analysis show TsWRKYs may multi-genes co-participate in regulating terpenoid biosynthesis. The findings revealed that TsWRKYs potentially play a regulatory role in secondary metabolite synthesis, forming the basis for further functional characterization of WRKY genes with the intention of improving T. sinensis

Arabidopsis Hormone Database: a comprehensive genetic and phenotypic information database for plant hormone research in Arabidopsis

Plant hormones are small organic molecules that influence almost every aspect of plant growth and development. Genetic and molecular studies have revealed a large number of genes that are involved in responses to numerous plant hormones, including auxin, gibberellin, cytokinin, abscisic acid, ethylene, jasmonic acid, salicylic acid, and brassinosteroid. Here, we develop an Arabidopsis hormone database, which aims to provide a systematic and comprehensive view of genes participating in plant hormonal regulation, as well as morphological phenotypes controlled by plant hormones. Based on data from mutant studies, transgenic analysis and gene ontology (GO) annotation, we have identified a total of 1026 genes in the Arabidopsis genome that participate in plant hormone functions. Meanwhile, a phenotype ontology is developed to precisely describe myriad hormone-regulated morphological processes with standardized vocabularies. A web interface (http://ahd.cbi.pku.edu.cn) would allow users to quickly get access to information about these hormone-related genes, including sequences, functional category, mutant information, phenotypic description, microarray data and linked publications. Several applications of this database in studying plant hormonal regulation and hormone cross-talk will be presented and discussed

Broadband Linear-Dichroic Photodetector in a Black Phosphorus Vertical p-n Junction

The ability to detect light over a broad spectral range is central for practical optoelectronic applications, and has been successfully demonstrated with photodetectors of two-dimensional layered crystals such as graphene and MoS2. However, polarization sensitivity within such a photodetector remains elusive. Here we demonstrate a linear-dichroic broadband photodetector with layered black phosphorus transistors, using the strong intrinsic linear dichroism arising from the in-plane optical anisotropy with respect to the atom-buckled direction, which is polarization sensitive over a broad bandwidth from 400 nm to 3750 nm. Especially, a perpendicular build-in electric field induced by gating in black phosphorus transistors can spatially separate the photo-generated electrons and holes in the channel, effectively reducing their recombination rate, and thus enhancing the efficiency and performance for linear dichroism photodetection. This provides new functionality using anisotropic layered black phosphorus, thereby enabling novel optical and optoelectronic device applications.Comment: 18 pages, 5 figures in Nature Nanotechnology 201

Upper ocean biogeochemistry of the oligotrophic North Pacific Subtropical Gyre : from nutrient sources to carbon export

Subtropical gyres cover 26–29% of the world’s surface ocean and are conventionally regarded as ocean deserts due to their permanent stratification, depleted surface nutrients, and low biological productivity. Despite tremendous advances over the past three decades, particularly through the Hawaii Ocean Time-series and the Bermuda Atlantic Time-series Study, which have revolutionized our understanding of the biogeochemistry in oligotrophic marine ecosystems, the gyres remain understudied. We review current understanding of upper ocean biogeochemistry in the North Pacific Subtropical Gyre, considering other subtropical gyres for comparison. We focus our synthesis on spatial variability, which shows larger than expected dynamic ranges of properties such as nutrient concentrations, rates of N2 fixation, and biological production. This review provides new insights into how nutrient sources drive community structure and export in upper subtropical gyres. We examine the euphotic zone in subtropical gyres as a two-layered vertically structured system: a nutrient-depleted layer above the top of the nutricline in the well-lit upper ocean and a nutrient-replete layer below in the dimly lit waters. These layers vary in nutrient supply and stoichiometries and physical forcing, promoting differences in community structure and food webs, with direct impacts on the magnitude and composition of export production. We evaluate long-term variations in key biogeochemical parameters in both of these euphotic zone layers. Finally, we identify major knowledge gaps and research challenges in these vast and unique systems that offer opportunities for future studies

Failure Analysis of the Guyed-V Tower Link Plate Deformation of a 500 kV Transmission Line

Component analysis, mechanical property test, field survey and finite element mechanical calculation were used to analyse and evaluate the deformation failure of the Guyed-V Tower link plate of a 500 kV transmission line. The results show that the main cause of the failure is the relaxation of the guy after right front guy or wire is subjected to a large impact towards the large side. The weak design of the link plate structure and the misfitting of angle steel are the indirect causes of the failure. The construction angle of anchor cable is larger than the design value, and the tension of anchor cable is low, which has a certain impact

Failure Analysis of the Strain Clamp Steel Anchor Corrosion of a 500 kV Transmission Line

By macroscopic observation, composition analysis, mechanical properties test, SEM, energy spectrum, overall grip tensile test and finite element mechanics calculation method, the strain clamp steel anchor corrosion failure of a 500 kV transmission line is analysed. The results show that the corrosion of steel anchor for 31 years are the result of the natural corrosion, steel anchor corrosion rate is accelerated due to the galvanic corrosion, crevice corrosion, stress and damaged surface of the galvanized layer, and achieve C4 environment (zinc 2.1–4.2 um per year, carbon steel 50–80 um per year). The hardware are qualified. It is suggested to replace the steel anchor with yellow rust on the surface after 5 years, and to reevaluate the next maintenance cycle of the gold fittings without yellow rust on the surface at present, and replace it when the corrosion depth reaches 10%. Before replacement, all fittings should be monitored, especially steel anchor

Effect of Gradient Heat Conduction on Secondary Recrystallization of Grain-Oriented Silicon Steel

The grain-oriented silicon steels were subjected to gradient heat conduction during high-temperature annealing by using thermal insulation cotton. The macrostructures of samples subjected to circumferential gradient heat conduction showed a “petal-like” morphology with peripheral columnar grains and central equiaxed grains, while samples subjected to transverse gradient heat conduction showed a morphology with approximately 50% columnar grains and 50% equiaxed grains. The grain orientations, magnetic domains as well as magnetic properties in different regions were detected. Results showed that the magnetic induction intensity of cylindrical grains was better than that of equiaxed grains while the iron loss was worse, which indicated that a fast heating rate during high-temperature annealing was conducive to the accuracy of Goss grains. The magnetic domains in columnar grains were wider than the equiaxed grains, which resulted in poorer iron loss. A theory of the competitive growth among secondary Goss grains was proposed. Under the condition of gradient heat conduction, once the Goss grains with the fastest heat conduction grew up abnormally, they would compete with other Goss grains which were supposed to survive in traditional processes and swallow up them until adjacent to the secondary equiaxed grains which were later developed

The inspection and strength checking of old circular prestressed reinforced concrete pole

The capacity expansion of the old transmission lines will lead to an increase in the load level of exsiting prestressed reinforced concrete pole. In this work, the structural parameters of old pole were detected by X-ray imaging technology. The force conditions of the modified concrete pole under the design conditions and actual service conditions were analyzed by Midas/civil. And combined with the calculation of bearing capacity of the circular prestressed reinforced concrete section, the strength checking was conducted. The results showed that X-ray imaging technology can be used to detect the number of steel bars in pole. In addition, the reforming scheme that existing prestressed reinforced concrete poles were used and original LGJ70/10 conductors were replaced with LGJ95/15, still possess considerable safety factor under the actual maximum load conditions